Dietary Effects of Tiger Nut (Cyperus esculentus) on Egg and Milt Quality of African Catfish (Clarias gariepinus) Broodstocks (Burchell, 1822)

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Adekunle Ayokanmi Dada
Gbajuola Gideon Oluwagbohunmi
Adedapo Adejoke Adeola
Oke Israel Opeyemi


The dietary effect Tiger nut (Cyperus esculentus) seed powder was examined on the egg and milt quality of African catfish broodstock.45 Female catfish broodstock of average body weight, 475 ± 25.00g and male catfish broodstock of average body weight, 487.09±13.57 g. Five diets with crude protein of 40% were formulated with different inclusion levels of Cyperus esculentus seed powder; 0, 0.5, 1.0, 1.5, 2.0 g per 100 g of experimental diet (D1, D2 ,D3, D4 and D5). The experiment was carried out for 70 days and fish fed twice daily at 3% body weight. Fish fed experimental diets showed significantly improved growth performance and reproductive indices over the control treatment. Fish fed D3 had the highest weight gain (173.6±10.98) in female catfish while D5 had highest weight gain (215.17±11.62) in male catfish. Fish fed with D4 had the highest percentage fertilization (93.00±3.05) and percentage hatchability (85.00±2.64) in female catfish while in male catfish the highest percentage fertilization (94.00±1.29) and hatchability (79.17±10.09) was from D5. Percentage survival and gonadosomatic index was higher in fish fed D5 in both female and male catfish. Histology of fish fed with Cyperus esculentus diets revealed oocytes formation within the ovaries. Ovary of fish fed D1 showed primary oocytes, with primary yolk accumulation in the ovary of fish fed D2 and there were improvement in the oocytes. Fish fed D3, D4 and D5 showed more matured oocytes and ripe yolk of late maturing stage. There was reduced seminiferous tubular lumen and scanty spermatozoa in D1 and D2 and there was high spermatozoa density in the seminiferous lumen seen in the testicular histology of fish fed diets D3, D4 and D5. This result revealed that dietary inclusion of Cyperus esculentus seed powder enhance growth and improves fertility in Clarias gariepinus which will improve the quantitiy of its seed production.

Cyperus esculentus, egg quality, sperm quality, fertility, Clarias gariepinus.

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Ayokanmi Dada, A., Oluwagbohunmi, G. G., Adejoke Adeola, A., & Israel Opeyemi, O. (2019). Dietary Effects of Tiger Nut (Cyperus esculentus) on Egg and Milt Quality of African Catfish (Clarias gariepinus) Broodstocks (Burchell, 1822). Asian Journal of Fisheries and Aquatic Research, 5(1), 1-12.
Original Research Article


Moses BS. Fisheries and ecotourism: A tool for National Development. Proceedings of the Fisheries Society of Nigeria (FISON) (Enim UM, Chukwu EI; Ajah PO, Aina-Abasi DA, Nwowu FM). 2006;412.

Gabriel VU, Akinrotimi OA, Bekibele DO, Anyanwu PE, Onunkwo DN. Economic benefit and ecological efficiency of integrated Fish farming in Nigeria. Scientific Research and Essay. 2007;2(8): 302 - 308.

Alatise OM. Fish breeding and fish multiplication, proceeding of the National fish farmers’ workshop. (Edited by AM Ajana). 1998;117-132.

Dada AA, Fagbenro D. Catfish fingerlings production in Nigeria. Proceedings of the 4th Annual Conference of School of Agriculture and Agricultural Technology, Federal University of Technology, Akure. 2008;107 -110.

Ayinla OA, Akande GR. Growth response of Clarias gariepinus (Burchell 1822) on silage-based diets. NIOMR Technical. Nigerian Institute for Oceanography and Marine Research, Lagos. 1988;19.

Owodeinde FG, Ndimele PE. Survival, growth and feed utilization of two clariid catfish (Clarias gariepinus, Burchell 1822 and Heterobranchus bidorsalis, Geoffroy, 1809) and their reciprocal hybrids. Journal Applied Ichthyology. 2011;27:1249-1253.

FAO. United nations food and agriculture organization. The state of World Fisheries and Aquaculture. FAO, Rome. 2006;142.

Agbai O, Nwanegwo CO. Effect of Methanolic Extract of Cyperus esculentus (Tiger nut) on Luteinizing Hormone, Follicle Stimulating Hormone, Testosterone, Sperm Count and Motility in Male Albino Wistar Rats. Journal of Medical and Applied Biosciences. 2011; 154.

Yakubu MT, Akanji MA. Effect of aqueous extract of Massularia acuminate stemon sexual behavior of male wistarrats. Evid Based Complement Alternative Medcine. 2011;738103.

AOAC. Association of official analytical chemists official method of analysis (19th Edition) (K. Heltich Edition), Arlington, Virginia. 2005;1:18-32.

Bagenal T. Aspects of fish fecundity. In ecology of fresh water fish production. 2nd edn, (Shelby DG ed.). Black well: Oxford, London. 1978;101-135.

Mims SD. Evaluation of activator solutions, motility duration and short-term storage of paddle fish spermatozoa. Journal of World Aquacultural Society. 1991;22:224-229.

Canyurt MA, Akhan S. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal of Fisheries Aquatic Science. 2008;8:171-175.

Rainis S, Mylonas C, Kyriakou Y, Divanach P. Enhancement of spermiation in European sea bass (Discentrarchus labrax) at the end of reproductive season using GnRHa implants. Aquaculture. 2003;19:873-890.

King M. Fisheries biology, assessment and management blackwell science, London. 1995;341.

Krause WJ. The art of examining and interpreting histologic preparation: A student handbook. 20 Partheton Publishing Group, UK. 2001;9-10.

APHA. Standard Method for Examination of water and waste water.21stEdition, American public Health Association, Washington D.C. 2005;776.

Viveen WJ, Ritcher CJJ, Van Oordt PGWJ, Janseen JAL, Huisman EA. Practical manual for culture of African Catfish. C. gariepinus. Directorate General for International Co-operation. The Hugue. P.93. 1986;3(3):27-38.

Al-Shaikh MN, Abdul Wahab TA, Abdul Kareem SH, Hamoudi SR. Protective effect of chufa tubers (Cyperus esculentus) on induction of Sperm abnormalities in mice treated with lead acetate. International Journal of Drug. Development Resources. 2013;5:387–92.

Amaal AM, Essraa MA. The effect of Cyperus esculentus on sperm function Parameters in prepubertal mice as a model for human. Journal of Science. 2010;7:389-393.

Adeparusi EO, Dada AA, Alale OV. Effect of medicinal plant (Kigelia africana) on sperm quality of African catfish, Clarias gariepinus (Burchell, 1822) broodstock. Journal of Agricultural Science. 2010;2(1): 193–199.

Dada AA, Ogunduyile FD. Effects of Velvet Bean (Mucuna pruriens) on sperm Quality of African Catfish Clarias gariepinus (bruchell, 1822) broodstock. Journal of Aquatic Science. 2011;6(6):655-661.

Dada AA, Aguda OE. Dietary effects of African walnut (Tetracarpidium conophorum) on the reproductive indices in male African catfish (Clarias gariepinus) broodstock. Journal of Aquatic Sciences. 2015;30(1A):107-118.

Mason D. Tiger Nuts; 2005.
Available: vegetables 1/ tiger nut.php
(Accessed December, 2009)

Tiger nut Traders Tiger nut and health; 2005.
(Accessed November 2009)

Carillo M, Sanuy S, Prat F, Cerda J, Ramos J, Mananos E, Bromage N. Sea Bass (Dicentrarchus labrax L). In: Broodstook management and egg and larval quality, (Eds: Bromage and J Roberts), Blackwell Science, Osmead, Oxford. 1995;424.

Ejete-Iroh VC, Dada AA. Dietary effects of fluted pumpkin (Telfairia occidentalis) leaf extract powder on male reproductive indices of African catfish (Clarias gariepinus) broodstock. Journal of Aquatic Sciences. 2015;31(1A):75-84.

Ibim AT, Sikoki FD. Effect of protein level on gonadal development of the African catfish. Journal of Biology, Agriculture and Healthcare. 2014;4(1):51–56.

Adewumi AA, Olaleye VF, Adesulu EA. Egg and sperm quality of the African catfish, Clarias gariepinus (Burchell) broodstock fed differently heated soybean – Base diets. Research Journal of Agriculture and Biological Sciences. 2005; 1(1):17–22.

Sule OD, Adikwu IA. Effect of broodstock size on egg and the African catfish, Clarias gariepinus under laboratory conditions. Journal of Aquatic Science. 2004;19(1):1– 4.

Dada AA, Ajilore VO. Use of ethanol extracts of Garcinia kola as fertility enhancer in female catfish Clarias gariepinus broodstock. International Journal of Fisheries and Aquaculture. 2009;1:1-5.

Dada AA. Effects of Garcinia kola seed meal on egg quality of the North African catfish, Clarias gariepinus (Burchell, 1822) broodstock. African Journal of Food, Agriculture, Nutrition and Development. 2012;12:6447-6459.

Bhatnagar A, Jana SN, Garg SK, Patra BC, Singh G, Barman UK. Water quality management in aquaculture. In: Course manual of summer school on development of sustainable aquaculture technology in fresh and saline waters, CCS Haryana agricultural, Hisar (India). 2004;203–10.

APHA. Standard method for examination of water and waste water. 17th edition, American Public Health Association, Washington D.C. 2002;1268.

Ekubo AA, Abowei JFN. Review of some water quality management principles in culture fisheries, Research Journal of Applied Sciences, Engineering and Technology. 2011;3(2):1342-1357.

Iwamatsu T, Ishijima S, Nakashima S. Movement of spermatozoa and changes in micropyles during fertilization in medaka eggs. Journal of Experimental Zoology. 1993;57–64.

Agbabiaka LA, Ezeafulukwe CF. Utilization of tigernut (Cyperus esculentus L.) meal as dietary supplement by African catfish (Clarias gariepinus Burchell, 1822) Fisheries and Aquaculture Journal. 2013; 64:1–7.

Sharma V, Thakur M, Chauhan NS, Dixit VK. Evaluation of the Anabolic, aphrodisiac reproduction activity of Anacyclus pyrethrum in Male Rats. Scientia Pharmaceutical. 2008;200:1-13.

Ayoola PB, Onawumi OO, Faboya OP. Chemical evaluation and nutritive values of Tetracarpidium conophorum (Nigerian walnut) seeds. Journal of Pharmacological Biomedical Sciences. 2011;11(15):1-5.

Fukuchi S, Hamaguchi K, Seike M, Himeno K, Sakata T, Yoshimatsu H. Role of fatty acid composition in the development of metabolic disorders in sucrose-induced obese rats. Experimental Biology and Medicine (Maywood). 2004; 229(6):486-93.